Advertiment
Baum CE, Ockers SB, English BC, Price DK, Sartor O, Figg WD.
Molecular Pharmacology Section, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA.
The androgen receptor gene (AR) plays an important role in molecular signaling and regulation and the subsequent cellular growth of prostate cancer. In addition, it is a highly variable region of the genome. We used direct nucleotide sequencing to genotype the entire exogenous coding region of the androgen receptor in ten commonly used prostate cancer cell lines. Our analysis confirmed the presence or absence of several known SNPs in the cell lines studied. We also assayed the number of CAG-repeat and GGC-repeat sequences for each for the ten cell lines. Our analysis identified three new mutations, one each in the DU145, LnCAP and RWPE-2 cell lines. In DU145, the DNA isolated in our lab was heterozygous at G527G (T>C transition), a polymorphism not previously reported. The LnCAP cells cultured in our lab were found to have a T>C transition (heterozygous), resulting in a S641P change that was not present in the ATCC cell line DNA. Lastly, a homozygous G>T transversion was found in RWPE-2 cells, resulting in the S187I change. This is potentially significant for use in cell culture and future cell model development.
Cancer Biol Ther. 2010 Mar 8;9(5).
Related Search
androgen receptor sequencing price,
Leave a Reply
Suggested Posts
Potential Strategy Aims To Stimulate The Immune System To Defeat Cancers Baum CE, Ockers SB, E glish BC, Price DK, Sartor O, Figg WD. Molecular Pharmacology Sectio , Ce ter for Ca cer Research, Natio al Ca cer I stitute, NIH, Bethesda, MD, USA. The a droge receptor ge e (AR) plays a importa t role i molecular sig ali g a d regulatio a d the subseque t cellular growth of prostate ca cer. I additio , it is a highly variable [...]. Posted March 20th, 2010 | 3:40 am
Smoking May Be Discouraged By Population-Specific Community-Based Cancer Screening Baum CE, Ockers SB, E glish BC, Price DK, Sartor O, Figg WD. Molecular Pharmacology Sectio , Ce ter for Ca cer Research, Natio al Ca cer I stitute, NIH, Bethesda, MD, USA. The a droge receptor ge e (AR) plays a importa t role i molecular sig ali g a d regulatio a d the subseque t cellular growth of prostate ca cer. I additio , it is a highly variable [...]. Posted March 20th, 2010 | 3:40 am
Breakthrough In Loading Gold Nanorods Into Cells Could Lead To New Cancer Treatment Baum CE, Ockers SB, E glish BC, Price DK, Sartor O, Figg WD. Molecular Pharmacology Sectio , Ce ter for Ca cer Research, Natio al Ca cer I stitute, NIH, Bethesda, MD, USA. The a droge receptor ge e (AR) plays a importa t role i molecular sig ali g a d regulatio a d the subseque t cellular growth of prostate ca cer. I additio , it is a highly variable [...]. Posted March 20th, 2010 | 3:40 am
Scientists Discover Post-Production Addition To Most Proteins Can Serve As A Key To Mediate Protein Interactions, Which Are At The Foundation Of Life Baum CE, Ockers SB, E glish BC, Price DK, Sartor O, Figg WD. Molecular Pharmacology Sectio , Ce ter for Ca cer Research, Natio al Ca cer I stitute, NIH, Bethesda, MD, USA. The a droge receptor ge e (AR) plays a importa t role i molecular sig ali g a d regulatio a d the subseque t cellular growth of prostate ca cer. I additio , it is a highly variable [...]. Posted March 20th, 2010 | 3:40 am
Risk of second cancer in cancer survivors mainly confined to the same cancer type as the first Baum CE, Ockers SB, E glish BC, Price DK, Sartor O, Figg WD. Molecular Pharmacology Sectio , Ce ter for Ca cer Research, Natio al Ca cer I stitute, NIH, Bethesda, MD, USA. The a droge receptor ge e (AR) plays a importa t role i molecular sig ali g a d regulatio a d the subseque t cellular growth of prostate ca cer. I additio , it is a highly variable [...]. Posted March 20th, 2010 | 3:40 am